Legionella pneumophila — The causative agent of Legionnaires’ disease

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منابع مشابه

Isolation of Legionella Pneumophila Serogroups 1, 8 & 10 (Causative Agents of Legionnaires\' Disease) from Water Sources in Tehran, Iran

In this survey, 187 samples collected from 9 different sources of water in Tehran such as: water cool‌ers, neonates incubators, water bathes (37° C), little pools in parks or squares, cooling towers, hot or cold water taps, were examined for Legionnaires' disease agents. Inoculating water samples on selec‌tive and nonselective media resulted in isolation of several strains of gram negative bact...

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Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes.

We have studied the interaction between virulent egg yolk-grown Legionella pneumophila Philadelphia 1 and human blood monocytes in vitro. The leukocytes were cultured in antibiotic-free tissue culture medium supplemented with 15% autologous human serum.L. pneumophila multiplied several logs, as measured by colony-forming units, when incubated with monocytes or mononuclear cells; the mid-log pha...

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Isolation and characterization of the cytoplasmic and outer membranes of the Legionnaires' disease bacterium (Legionella pneumophila)

Legionella pneumophila, the etiologic agent of Legionnaires' disease, is phagocytized in an unusual way and multiplies in human mononuclear phagocytes in a novel phagosome. As a first step toward understanding these L. pneumophila-phagocyte interactions, we have studied the envelope of L. pneumophila Philadelphia 1 strain. We isolated cell envelopes by treating whole bacterial cells with lysozy...

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Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes

Previous studies have shown that L. pneumophila multiplies intracellularly in human monocytes and alveolar macrophages within a membrane-bound cytoplasmic vacuole studded with ribosomes. In this paper, the formation of this novel vacuole is examined. After entry into monocytes, L. pneumophila resides in a membrane-bound vacuole. During the first hour after entry, vacuoles containing L. pneumoph...

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The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes

The interactions between the L. pneumophila phagosome and monocyte lysosomes were investigated by prelabeling the lysosomes with thorium dioxide, an electron-opaque colloidal marker, and by acid phosphatase cytochemistry. Phagosomes containing live L. pneumophila did not fuse with secondary lysosomes at 1 h after entry into monocytes or at 4 or 8 h after entry by which time the ribosome-lined L...

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ژورنال

عنوان ژورنال: Progress In Microbes & Molecular Biology

سال: 2021

ISSN: 2637-1049

DOI: 10.36877/pmmb.a0000193